DAF-2 (DMSO solution) is widely used in biological and medical research as a fluorescent probe for the detection of nitric oxide (NO) in living cells and tissues. Its application is particularly significant in studying the role of NO in various physiological and pathological processes, such as vascular function, immune response, and neurotransmission. The DMSO solution form ensures effective penetration into cells, allowing for real-time monitoring of NO levels. Researchers utilize DAF-2 to investigate oxidative stress, inflammation, and cardiovascular diseases, providing valuable insights into cellular signaling mechanisms. Its high sensitivity and specificity make it a crucial tool in understanding NO-related pathways and developing therapeutic strategies.