DBO-83 is widely used as a fluorescent dye in various scientific and industrial applications. Its high photostability and strong fluorescence make it ideal for use in bioimaging, where it helps in visualizing cellular structures and processes with high precision. In molecular biology, it is employed as a labeling agent for tracking biomolecules, such as proteins and nucleic acids, during experiments. Additionally, DBO-83 is utilized in the development of optical sensors due to its sensitivity to environmental changes, enabling the detection of specific analytes in complex mixtures. Its application extends to material science, where it is incorporated into polymers and coatings to create advanced fluorescent materials for security markings and anti-counterfeiting measures.