Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology. It enables the tagging of proteins, peptides, and other biomolecules for visualization and tracking in cellular and molecular studies. Its coumarin derivative provides strong fluorescence, making it ideal for fluorescence microscopy, flow cytometry, and other imaging techniques. The succinimidyl ester group allows for efficient conjugation to primary amines, ensuring stable and specific labeling. It is particularly useful in studying protein interactions, localization, and dynamics in live or fixed cells. Additionally, it is employed in the development of fluorescent probes and sensors for detecting biological processes and environmental changes.