Acridine Orange，AO
biotech grade
science Other reagents with same CAS 10127-02-3
blur_circular Chemical Specifications
description Product Description
Acridine Orange is widely used in biological and medical research for staining nucleic acids, particularly in fluorescence microscopy. It binds to DNA and RNA, emitting different fluorescence colors—green for DNA and red or orange for RNA—making it valuable for distinguishing between the two in cellular studies. This property is especially useful in detecting apoptosis, as it helps identify changes in nuclear morphology. Additionally, it is employed in microbiology to assess bacterial viability by differentiating live and dead cells based on membrane integrity. In clinical diagnostics, Acridine Orange aids in rapid screening of blood samples for malaria parasites and other infections due to its ability to highlight nucleic acids in pathogens. Its versatility also extends to environmental studies, where it is used to analyze microbial populations in water and soil samples.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Loss on Drying (105°C/1 h) | 0-3% |
| Appearance | Brown to red-brown powder |
| Identification (IR) | CONFORMS TO STANDARD |
| Solubility (c = 1 mg/ml H2O) | Clear orange to brown solution |
| Extinction Coefficient (0.005 g/l; H2O) | ≥38000 (Wavelength: 489-495 nm) |
| Extinction Coefficient (0.005 g/l; H2O) | ≥ 18000 (Wavelength: 285-291 nm) |
| Extinction Coefficient (0.005 g/l; H2O) | ≥38000 (Wavelength: 265-271 nm) |
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