5-Isothiocyanato fluorescein
Isomer I Biotech Grade
Reagent
Code: #50763
Alias
5-FITC
CAS Number
3326-32-7
blur_circular Chemical Specifications
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Molecular Information
Weight
389.38 g/mol
Formula
C₂₁H₁₁NO₅S
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Registry Numbers
EC Number
222-042-0
MDL Number
MFCD00005063
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Physical Properties
Melting Point
>360 °C(lit.)
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Storage & Handling
Storage
room temperature
description Product Description
Used extensively in biological research as a fluorescent labeling agent. It binds to proteins, antibodies, and other biomolecules, enabling their detection and visualization under fluorescence microscopy. Commonly applied in immunofluorescence assays, flow cytometry, and cell imaging studies. Its isothiocyanate group reacts with amino groups in biomolecules, forming stable thiourea bonds. This makes it a valuable tool for tracking cellular processes, protein interactions, and molecular localization in live or fixed cells. Also utilized in diagnostic applications to identify specific targets in complex biological samples.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Purity | 98-100% |
| Appearance | Yellow to orange powder and/or chunks |
| Solubility | Clear, Yellow to Very Dark Yellow and Green-Yellow to Very Dark Green-Yellow, 1 mg/mL in Acetone |
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