Used extensively in fluorescence microscopy and bioimaging due to its strong fluorescence properties. It serves as a fluorescent probe for labeling biomolecules, enabling visualization of cellular structures and processes. Commonly applied in live-cell imaging, it helps track molecular interactions and dynamics within cells. Its photostability and sensitivity make it suitable for detecting low-abundance targets in complex biological systems. Additionally, it is utilized in flow cytometry for cell sorting and analysis, as well as in biochemical assays for studying enzyme activity and protein interactions. Its compatibility with various labeling techniques enhances its versatility in research and diagnostic applications.