Used as a fluorescent probe in biochemical and analytical applications due to its strong emission properties in aqueous solutions. It is particularly effective in tracking molecular interactions and monitoring conformational changes in proteins and nucleic acids. Its sulfonate group enhances water solubility and reduces nonspecific binding, making it suitable for use in biological imaging and diagnostic assays. Also employed in micelle and membrane studies because of its sensitivity to environmental polarity. Stable under physiological pH conditions, it is compatible with live-cell imaging and real-time monitoring systems.