Widely used in molecular biology laboratories for nucleic acid detection due to its strong fluorescent properties when bound to DNA or RNA. It is commonly employed in agarose gel electrophoresis to visualize DNA bands under ultraviolet light. The compound integrates between nucleic acid base pairs, enhancing fluorescence and allowing researchers to monitor the progress of DNA separation and estimate fragment sizes. Due to its high sensitivity, it is effective at low concentrations in staining gels during genetic analysis, PCR product verification, and restriction enzyme digestion checks. Safe handling and proper disposal are essential due to its mutagenic nature.