Used primarily in research as a fluorescent probe for DNA detection due to its strong intercalating properties with nucleic acids. Exhibits enhanced fluorescence upon binding to double-stranded DNA, making it valuable in biochemical assays for monitoring DNA interactions and structural changes. Also investigated for potential applications in photodynamic therapy and as a sensor in molecular biology for detecting specific DNA sequences. Its nitro groups contribute to electron-accepting characteristics, useful in studies involving electron transfer in biological systems.