Widely used in bioimaging and molecular diagnostics, this chemical serves as a fluorescent label for biomolecules such as proteins, nucleic acids, and antibodies. Its strong absorption in the visible range and bright emission make it ideal for fluorescence microscopy, flow cytometry, and microarray analysis. The carboxylic acid group allows easy conjugation to amino groups via EDC coupling, enabling stable linkage to biomolecules. It is especially useful in dual-labeling experiments due to its distinct spectral properties, allowing clear differentiation from other fluorophores. Its moderate hydrophilicity supports good solubility in aqueous buffers, enhancing performance in biological assays.