Used primarily as a fluorescent probe in biochemical and cellular assays due to its high sensitivity and photostability. Its carboxy and methoxy groups enhance water solubility and allow for easy conjugation to biomolecules such as antibodies, proteins, and nucleic acids. Commonly employed in fluorescence microscopy, flow cytometry, and immunoassays for detecting specific targets in complex biological samples. The dichloro substitution increases fluorescence quantum yield and improves resistance to photobleaching, making it suitable for long-term imaging experiments. Also utilized in pH sensing applications because of its fluorescence intensity changes in response to environmental pH shifts.