Widely used as a derivatization reagent in analytical chemistry, particularly for the detection and quantification of primary and secondary amines in biological samples. It reacts selectively with amine groups to form highly fluorescent derivatives, making it valuable in high-performance liquid chromatography (HPLC) with fluorescence detection. Commonly applied in the analysis of amino acids, peptides, neurotransmitters, and pharmaceuticals in urine, plasma, and tissue extracts. Its high reactivity and sensitivity allow for trace-level detection, useful in clinical diagnostics and pharmacokinetic studies. Also employed in the synthesis of fluorescent probes and sensors due to its electron-deficient aromatic structure and ability to participate in nucleophilic substitution reactions.