Widely used as a fluorescent dye in biomedical and analytical applications, this compound is especially valuable in real-time PCR (qPCR) due to its bright fluorescence and photostability. It serves as a reporter dye in probe-based assays, such as TaqMan, where it enables accurate detection and quantification of nucleic acids. Its distinct emission spectrum allows for efficient multiplexing when combined with other fluorophores. Additionally, it is employed in fluorescence microscopy and flow cytometry for labeling and tracking biomolecules. The single isomer form ensures consistent performance and reduced variability in sensitive detection systems.