Used in bioconjugation for labeling biomolecules such as proteins, peptides, and nucleic acids due to its bright near-infrared fluorescence. Commonly applied in fluorescence imaging, flow cytometry, and immunohistochemistry to enable detection and tracking in biological systems with minimal background interference. Ideal for live-cell imaging because of its cell permeability and stability. Also employed in developing diagnostic assays and molecular probes where sensitive detection is required.