Used as a fluorescent probe in biochemical assays due to its coumarin core, enabling detection of enzyme activity and molecular interactions in cellular environments. Its fluorobenzoyl group enhances binding selectivity toward specific esterases and proteases, making it valuable in drug metabolism studies. Also employed in imaging applications for real-time monitoring of hydrolysis reactions in live cells. Shows potential in targeted drug delivery systems where controlled release is triggered by enzymatic cleavage of the glycinate ester bond.