Used for long-term tracing of cell membranes in live cells due to its lipophilic properties that allow stable integration into the lipid bilayer. Commonly applied in cell migration, proliferation, and fusion studies. Fluorescent signal is retained over multiple cell divisions, making it suitable for developmental biology and cancer research. Also used in co-culture systems to distinguish between different cell populations. Compatible with live-cell imaging and can be combined with other fluorescent markers for multiplex assays. Stable in fixed tissues, allowing post-imaging immunostaining or preservation for later analysis.