Used primarily as a fluorescent probe in biological imaging and cellular assays. Its structure allows it to bind selectively to specific cellular components, making it valuable for tracking membrane dynamics and studying receptor localization. The compound’s fluorescence properties change in response to environmental polarity, enabling researchers to monitor changes in cellular microenvironments. It is also employed in flow cytometry and confocal microscopy due to its strong emission signal and photostability. Its iodide salt form enhances solubility in aqueous buffers, facilitating use in live-cell imaging with minimal cytotoxicity at working concentrations.