Used as a fluorescent dye in biological imaging and staining applications. Its strong fluorescence and lipophilic nature make it suitable for membrane labeling in live and fixed cells. Commonly employed in neuroscience for tracing neuronal pathways due to its ability to diffuse along cell membranes and highlight axonal structures. Also utilized in histology for visualizing cellular morphology and in developmental biology for cell lineage tracing. The compound’s cationic structure enhances retention in mitochondria, allowing for mitochondrial network visualization in certain cell types.