Widely used as a fluorescent probe in biological imaging, this compound is particularly effective for detecting and quantifying amyloid fibrils and protein aggregates associated with neurodegenerative diseases such as Alzheimer's and Parkinson’s. Its sulfonate group enhances water solubility and cellular uptake, allowing for efficient staining in live and fixed cells. The benzo[d]thiazole and tetrazolium components contribute to strong fluorescence emission upon binding to β-sheet-rich structures, offering high signal-to-noise ratios in microscopy and flow cytometry. It is also employed in high-throughput screening assays to identify potential therapeutic agents that inhibit protein aggregation. Due to its specificity and photostability, it serves as a reliable tool in neuropathology research and drug development.