Used as a fluorescent probe in biological imaging due to its strong emission properties when bound to certain biomolecules. It selectively interacts with nucleic acids and is employed in studying DNA and RNA structures in cellular environments. Its chloroquinoline groups enhance cell membrane permeability, making it suitable for live-cell imaging.

Serves as a starting material in research and development of antimicrobial drugs, particularly against bacteria and drug-resistant parasites. It exhibits inhibitory effects on the growth of both Gram-positive and Gram-negative bacteria by disrupting DNA function and protein synthesis in microbial cells.

Studied as an inhibitor of enzymes associated with chronic diseases, such as topoisomerase involved in DNA replication, showing potential for development into anticancer therapeutics.

Also investigated for antimicrobial activity due to structural similarity to quinolone antibiotics. Shows potential in photodynamic therapy as a photosensitizer that generates reactive oxygen species under light exposure.

In materials science, it can be used as a ligand to form coordination compounds with special properties, such as luminescence or bioactivity, applicable in heavy metal sensors or targeted drug delivery systems.