Used as a fluorescent probe in biological imaging due to its high photostability and bright green emission. Ideal for labeling biomolecules such as proteins and nucleic acids in live-cell imaging. Its cell permeability allows for intracellular targeting without transfection agents. Commonly applied in fluorescence microscopy and flow cytometry for real-time tracking of cellular processes. Also utilized in energy transfer studies as a donor or acceptor fluorophore due to its narrow emission spectrum and high quantum yield.