Used as a fluorescent probe in biological imaging due to its strong near-infrared emission and high photostability. It selectively accumulates in mitochondria and cell membranes, making it valuable for tracking cellular dynamics and membrane potential changes. Commonly applied in live-cell imaging and apoptosis studies. Also employed in flow cytometry and confocal microscopy for real-time monitoring of cellular processes. Its sulfonate group enhances water solubility and reduces nonspecific binding, improving signal clarity in complex biological environments.