Used as a fluorescent probe for detecting hydrogen sulfide in biological systems. It selectively reacts with H₂S to produce a strong fluorescent signal, enabling real-time monitoring in live cells and tissues. Commonly applied in studies related to cellular signaling, oxidative stress, and sulfur metabolism. Also utilized in imaging applications due to its high sensitivity and low background fluorescence. Suitable for both in vitro and in vivo experiments where detection of trace H₂S is required.