Used as a fluorescent probe in biological imaging due to its strong emission properties in aqueous environments. It binds selectively to nucleic acids, making it valuable in DNA and RNA detection assays. Its ethoxy group enhances cell membrane permeability, allowing for live-cell staining without transfection agents. Also employed in photodynamic therapy research owing to its ability to generate reactive oxygen species under light exposure. Additionally, it serves as an intermediate in synthesizing antimalarial and antitumor agents due to its acridine core's affinity for intercalating into DNA.