Widely used as a fluorescent probe in biochemical and biophysical research, this compound is particularly valuable for studying protein conformational changes and detecting hydrophobic binding sites. It exhibits enhanced fluorescence in non-polar environments, making it ideal for monitoring interactions between proteins and ligands, especially in assays involving molten globule states or amyloid formation. Commonly employed in high-throughput screening for drug discovery, it helps identify molecules that alter protein folding or aggregation. Its sensitivity to environmental polarity also supports its use in membrane studies and enzyme activity assays.