Used extensively as a fluorescent probe to detect reactive oxygen species (ROS) in biological systems. It is particularly valuable in cell-based assays to measure oxidative stress, as it becomes highly fluorescent upon oxidation. Widely applied in research involving cellular metabolism, apoptosis, and studies on the effects of antioxidants. Also utilized in flow cytometry and microscopy to visualize and quantify ROS production in live cells. Its sensitivity and specificity make it a critical tool in understanding cellular responses to environmental and chemical stressors.