HADA is widely used in biological research as a fluorescent dye for labeling and tracking cellular processes. It is particularly effective in studying bone formation and mineralization, as it binds to newly formed hydroxyapatite crystals in bone tissue. This allows researchers to visualize and quantify osteoblast activity and bone growth in real-time. Additionally, HADA is utilized in live-cell imaging to monitor dynamic biological events, providing insights into tissue development and regeneration. Its compatibility with various imaging techniques makes it a valuable tool in both in vitro and in vivo studies.