Used as a fluorescent probe in biochemical research to study protein interactions and conformational changes. It binds to hydrophobic regions of proteins, enhancing fluorescence and allowing detection of structural alterations. Commonly applied in assays to monitor protein folding, unfolding, and aggregation. Also utilized in membrane studies to investigate lipid-protein interactions due to its sensitivity to environmental polarity. In addition, it serves as a tracer in electrophoresis to visualize protein bands. Its ability to act as a pH indicator in certain systems further extends its utility in analytical chemistry.