Resorufin is widely used as a fluorescent dye in various biochemical and cellular assays. Its strong fluorescence makes it valuable in detecting enzymatic activities, particularly in assays involving oxidoreductase enzymes like diaphorase or cytochrome P450. It is also employed in viability assays to measure cell metabolism, where the reduction of resazurin to resorufin indicates active cellular respiration. Additionally, resorufin is utilized in environmental monitoring to detect pollutants, as it can act as an indicator in redox reactions. Its applications extend to molecular biology, where it serves as a reporter molecule in fluorescence microscopy and flow cytometry studies.