NIAD-4 is primarily utilized in the field of biomedical research, particularly in the study of protein aggregation and neurodegenerative diseases. It serves as a fluorescent probe that binds specifically to amyloid fibrils, which are protein aggregates associated with conditions like Alzheimer's disease. This binding property allows researchers to visualize and monitor the formation and progression of amyloid plaques in cellular and tissue models. Additionally, NIAD-4 is employed in high-throughput screening assays to identify potential therapeutic compounds that can inhibit or disrupt amyloid aggregation. Its application extends to the development of diagnostic tools for early detection of amyloid-related pathologies, aiding in the advancement of treatments for neurodegenerative disorders.