3-hexanoyl-NBD Cholesterol is widely used in biological research to study cholesterol trafficking and distribution within cellular membranes. Its fluorescent NBD (nitrobenzoxadiazole) tag allows for real-time visualization and tracking of cholesterol movement in live cells. This compound is particularly valuable in investigating lipid rafts, membrane dynamics, and the role of cholesterol in cellular processes such as signaling and endocytosis. It is also employed in assays to examine the effects of cholesterol modulation in diseases like atherosclerosis and neurodegenerative disorders. Additionally, it serves as a tool to explore the interactions between cholesterol and proteins or other lipids in membrane environments.