NBD-H is widely used as a fluorescent labeling reagent in biochemical and analytical research. Its primary application is in the detection and quantification of carbonyl compounds, such as aldehydes and ketones, in various biological samples. The compound reacts selectively with these carbonyl groups to form highly fluorescent hydrazone derivatives, enabling sensitive and specific analysis.

In proteomics, NBD-H is employed to label and track proteins or peptides that contain carbonyl modifications, which are often indicative of oxidative stress or disease states. This makes it a valuable tool for studying protein oxidation and degradation pathways. Additionally, it is used in environmental analysis to detect trace levels of carbonyl pollutants in air, water, and soil samples.

The fluorescence properties of NBD-H derivatives allow for easy visualization and quantification using techniques like HPLC, fluorescence microscopy, and spectrophotometry. Its stability and high sensitivity make it a preferred choice for researchers studying oxidative damage, metabolic disorders, and environmental contamination.