This compound is primarily used in high-performance liquid chromatography (HPLC) as a labeling reagent. It reacts with specific functional groups in target molecules, enabling their detection and quantification with high sensitivity. The coumarin moiety provides strong fluorescence, making it ideal for trace analysis in complex mixtures. It is particularly useful in biological and environmental samples, where precise measurement of low-concentration analytes is required. The isocyanate group ensures efficient and selective binding to amines, alcohols, or other nucleophiles in the target molecules, enhancing labeling efficiency. Its application is critical in research fields such as proteomics, metabolomics, and pharmaceutical analysis.