Used as a fluorescent probe in biochemical and analytical applications due to its strong emission properties upon binding to specific biomolecules. It is commonly employed in protein labeling and detection, especially in immunoassays and cellular imaging, where its maleimide group reacts selectively with thiol groups (-SH) in cysteine residues, enabling site-specific conjugation. Its benzimidazole core enhances photostability and fluorescence quantum yield, making it suitable for real-time monitoring in live cells. Also utilized in the development of biosensors and in studying protein-protein interactions.