Used in bioconjugation for labeling biomolecules such as proteins, peptides, and antibodies. The N-hydroxysuccinimide ester group reacts efficiently with primary amines to form stable amide bonds, making it ideal for attaching fluorescent tags in fluorescent labeling applications. The 7-methoxycoumarin moiety provides blue fluorescence upon excitation, useful in fluorescence microscopy, flow cytometry, and cellular imaging. Commonly employed in probe development for studying biological systems due to its good photostability and relatively low background fluorescence. Also used in the synthesis of fluorescent sensors and tracers for monitoring biochemical processes.