Used as a fluorescent labeling reagent in biochemical and analytical applications, particularly for detecting amines and thiols in proteins and peptides. Its high reactivity with nucleophiles allows for selective tagging in complex biological samples. The compound’s strong fluorescence upon conjugation makes it valuable in fluorescence microscopy, immunoassays, and high-performance liquid chromatography (HPLC) detection. Also employed in the synthesis of fluorescent sensors and probes due to its electron-deficient aromatic structure and photostability.