Used in biochemical research for labeling peptides and proteins to enhance detection in fluorescence-based assays. Its high sensitivity to environmental changes makes it valuable in studying protein folding, conformational changes, and enzyme activity. Commonly employed in HPLC and capillary electrophoresis as a fluorescent tag for amino acid and peptide analysis. Also applied in protease assay development, where cleavage of the labeled sequence produces a measurable fluorescent signal.