Dansylamide is a fluorescent sulfonamide compound used primarily as a probe in biochemical and biophysical studies. It is widely employed to investigate protein-ligand interactions, particularly binding to human serum albumin, where changes in its fluorescence intensity and wavelength upon binding allow for the determination of binding constants and sites. Additionally, it serves as a competitive inhibitor of carbonic anhydrase enzymes, aiding in enzyme kinetics and inhibition studies. Its high fluorescence quantum yield and sensitivity to environmental changes make it valuable in fluorescence spectroscopy for monitoring biomolecular conformational changes and in drug discovery research targeting protein binding pockets.