DAF-FM is widely used in biological and medical research as a sensitive fluorescent probe for the detection of nitric oxide (NO) in live cells and tissues. It is particularly valuable in studying the role of NO in various physiological and pathological processes, such as neurotransmission, immune response, and cardiovascular function. When DAF-FM reacts with NO, it produces a highly fluorescent compound, allowing researchers to visualize and quantify NO levels with high specificity and sensitivity. This makes it a crucial tool in understanding the dynamics of NO production and its impact on cellular signaling pathways. Additionally, DAF-FM is applied in drug development to assess the efficacy of compounds that modulate NO synthesis or activity, aiding in the discovery of therapies for conditions like hypertension, inflammation, and neurodegenerative diseases.