5(6)-Carboxyfluorescein, mixture of isomers
≥95% (HPLC), 5- and 6-isomer mixture
science Other reagents with same CAS 72088-94-9
blur_circular Chemical Specifications
description Product Description
5(6)-Carboxyfluorescein is widely used as a fluorescent tracer in biological and chemical research. Its high fluorescence intensity and water solubility make it ideal for tracking and visualizing processes in cells and tissues. It is commonly employed in cell viability assays, where it helps distinguish between live and dead cells based on membrane integrity. Additionally, it is used in fluorescence microscopy to study cell permeability, endocytosis, and intracellular pH changes. In molecular biology, it serves as a labeling agent for proteins, nucleic acids, and other biomolecules, enabling their detection and analysis. It is also utilized in microfluidic studies to monitor fluid flow and mixing dynamics. Its versatility and reliability make it a valuable tool in various scientific applications.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Purity (HPLC) | 95-100 |
| Appearance | Yellow to orange to brown powder |
| Fluorescence | Ex 492 nm; Em 517 nm in 0.1 M Tris pH 8.0 |
| Infrared Spectrum | Conforms to Structure |
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