Fluorescein o-acrylate is widely used in the development of fluorescent probes and sensors due to its ability to emit strong fluorescence under UV light. It is commonly incorporated into polymeric materials to create fluorescent polymers, which are applied in bioimaging and diagnostic assays. The compound is also utilized in the preparation of hydrogels and other biocompatible materials for tracking cellular processes and drug delivery systems. Additionally, it serves as a labeling agent in biochemical research to visualize and study molecular interactions in real-time. Its acrylate group allows for easy covalent bonding to various substrates, enhancing its versatility in scientific applications.