6-Aminofluorescein is widely used as a fluorescent dye in biological and chemical research. Its strong fluorescence makes it valuable for labeling and tracking molecules in various assays. In molecular biology, it is often employed in fluorescence microscopy to visualize cellular structures and processes. It is also utilized in flow cytometry to detect and analyze specific cell populations. Additionally, 6-Aminofluorescein is used in immunoassays and DNA sequencing as a marker to enhance detection sensitivity. Its application extends to environmental monitoring, where it helps trace pollutants and study water flow patterns. The dye’s ability to bind to proteins and nucleic acids further broadens its utility in biochemical studies.