This chemical is widely used as a fluorescent tracer in biological and medical research. It is particularly valuable for labeling proteins, antibodies, and other biomolecules, enabling their visualization under fluorescence microscopy. Its application extends to flow cytometry, where it helps in analyzing and sorting cells based on specific markers. Additionally, it is employed in immunohistochemistry to study tissue samples and detect the presence of specific antigens. The compound’s high fluorescence intensity and stability make it a preferred choice for tracking cellular processes, such as endocytosis and intracellular transport. It is also utilized in environmental studies to monitor water flow and contamination by acting as a tracer in hydrological systems.