Used extensively in biochemical and cellular research as a fluorescent probe for detecting and quantifying various biological processes. Its ability to bind to specific molecules makes it valuable in fluorescence microscopy and flow cytometry, enabling visualization of cellular structures and activities. Commonly applied in assays to measure enzyme activity, particularly proteases, by monitoring changes in fluorescence intensity. Also utilized in the study of cell viability and apoptosis, providing insights into cellular health and mechanisms of cell death. Its high sensitivity and specificity make it a crucial tool in diagnostic applications, including the detection of biomarkers in clinical samples. Additionally, employed in drug discovery to screen potential therapeutic compounds by observing their interactions with target proteins.