Used extensively in biochemical research as a fluorescent tracer and probe. Its high fluorescence intensity makes it ideal for labeling and tracking molecules in cellular imaging studies. Commonly applied in fluorescence microscopy to visualize cellular structures and processes. Also utilized in flow cytometry for analyzing cell populations based on fluorescence signals. In molecular biology, it serves as a marker in assays to detect enzyme activity or gene expression. Its stability and sensitivity in various pH conditions enhance its utility in diagnostic applications, such as detecting biomarkers in clinical samples. Additionally, it is employed in environmental monitoring to trace pollutants or study biological interactions in ecosystems.