Used primarily as a fluorescent dye in biological and chemical research. It is widely applied in fluorescence microscopy to stain and visualize cellular components, particularly in live-cell imaging due to its high sensitivity and stability. It is also utilized in flow cytometry to detect and quantify specific molecules within cells. Additionally, it serves as a pH indicator in various biochemical assays, helping to monitor changes in acidity or alkalinity in solutions. Its fluorescent properties make it valuable in diagnostic applications, such as detecting pathogens or analyzing DNA and protein interactions.