Used as a fluorescent tracer in biological and environmental studies to detect and measure reactive oxygen species (ROS) in cells. It is particularly valuable in assays for oxidative stress, where it helps to visualize and quantify the presence of hydrogen peroxide and other peroxides. Additionally, it serves as a pH indicator in certain biochemical experiments due to its pH-dependent fluorescence properties. In industrial applications, it is employed as a dye in flow visualization techniques to track fluid movement and mixing processes. Its high sensitivity and stability make it a preferred choice in various analytical and diagnostic procedures.