Used extensively as a fluorescent tracer in biological and environmental studies. It helps in tracking fluid flow and transport processes in various systems. In cell biology, it serves as a vital dye for monitoring cell viability and membrane integrity. It is also employed in fluorescence microscopy to visualize cellular structures and processes. Additionally, it plays a crucial role in assays for detecting enzyme activity and studying molecular interactions. Its high fluorescence intensity and stability make it a preferred choice in diagnostic applications and research.