Used extensively in molecular biology and biochemistry for labeling biomolecules, particularly proteins and nucleic acids. Its succinimidyl ester group facilitates covalent attachment to primary amines, enabling the creation of fluorescent conjugates. Commonly employed in fluorescence microscopy, flow cytometry, and immunoassays to visualize and track cellular components. Its high quantum yield and photostability make it suitable for sensitive detection applications. Additionally, it is utilized in fluorescence resonance energy transfer (FRET) studies to investigate molecular interactions and conformational changes.