Used extensively in medical diagnostics, particularly in ophthalmology to detect corneal abrasions, ulcers, and foreign bodies. It helps in evaluating the fit of contact lenses by highlighting areas of improper contact with the eye. In angiography, it is employed to visualize blood vessels in the retina, aiding in the diagnosis of conditions like diabetic retinopathy and macular degeneration. Additionally, it serves as a tracer in environmental studies to track water flow and contamination in rivers and groundwater. Its fluorescent properties make it valuable in various scientific research applications, including cellular imaging and flow cytometry.